Delineation of cellular stages and identification of key proteins for reduction and biotransformation of Se(IV) by Stenotrophomonas bentonitica BII-R7

The widespread use of selenium (Se) in technological applications (e.g., solar cells and electronic devices) has led to an accumulation this metalloid the environment toxic levels. newly described bacterial strain Stenotrophomonas bentonitica BII-R7 been demonstrated reduce mobile Se(IV) Se(0)-nanoparticles (Se(0)NPs) volatile species. Amorphous Se-nanospheres are reported aggregate form crystalline nanostructures trigonal selenium. We investigated molecular mechanisms underlying biotransformation less forms using differential shotgun proteomics analysis S. grown with or without sodium selenite for three different time-points. Results showed increase abundance several proteins involved reduction stabilization Se(0)NPs, such as glutathione reductase, bacteria Se(IV), addition many transport functions, including RND (resistance-nodulation-division) systems, possibly facilitating Se uptake. Notably oxidative stress defense catalase/peroxidase HPI) were also induced by exposure. Electron microscopy analyses confirmed amorphous nanospheres Se. Overall, our results highlight potential reducing bioavailability Se, which provides a basis both development bioremediation strategies eco-friendly synthesis biotechnological nanomaterials.